People Who Suffer From Insomnia Use It! How To Ensure The Quality of Its Medicine?

Semen Zizyphi Spinosae

 The jujube kernel for rhamliaceae plant jujube [Ziziphus jujuba Mill.var.spinosa（Bunge）Hu ex H.F. Chou] dry mature seeds, first contained in 'Shennong Materia Medica Classic', listed as the top grade, its taste sweet, sour, flat, liver, gallbladder, heart meridian, with the effect of calming the mind, nourishing the liver, collecting sweat and producing fluid.

Modern pharmacological studies show that jujube kernel has the functions of lowering blood pressure, anti-anxiety, anti-myocardial ischemia and treating neurasthenia. It is a common clinical drug in traditional Chinese medicine and is mainly produced in Hebei, Shandong, Liaoning, Shaanxi and other places. Jujube kernel is one of the common drugs for clinical treatment of insomnia, its effective ingredients spinolin and jujube kernel saponin A is the main component of its sedative, hypnotic effect, now listed pharmaceutical preparations have jujube kernel god capsule, compound jujube kernel soft capsule, etc., and in the treatment of insomnia, depression and other diseases has achieved significant curative effect.

 The demand for sour jujube kernel has increased

 According to the World Health Organization, the global rate of sleep disorders reaches 27%, making it the second most common mental disorder in the world. With the acceleration of people's life pace, the incidence of insomnia is becoming more and more high, and the clinical demand for jujube kernel also increases.

 Therefore, the quality of jujube kernel has also attracted much attention. Only from its appearance, color, mildew, moth, color, fullness, miscellaneous content, etc., etc., it is difficult to fully reflect the relationship between the quality and grade of jujube kernel, and the content of spinolin and saponin A should be determined.

Next INNOTEG will tell you how to use HPLC to accurately determine the pinolin in jujube kernel.

                                 How to determine the spinolin in the jujube kernel?

Sample Preparation

1. Take about 1g of powder (through screen 4), accurately;

2. Add petroleum ether (60~90℃);

3. Heat and reflux for 4 hours, discard the petroleum ether liquid, wave the solvent away from the residue, and transfer to the conical bottle;

4. Add 20ml of 70% ethanol, heat and reflux for 2 hours, and filter;

5. The filter residue is washed with 70% ethanol 5ml, combine the wash liquid and filtrate, and recover the solvent to dry;

6. Dissolve the residue with methanol, transfer to a 5ml measuring bottle, add methanol to the scale, shake well, filter, and take the continuous filtrate.

Chromatographic Condition

Column: INNOTEG YND-C18

Specifications: 4.6 * 2,505 μ m

Wavelength: 335nm

Mobile phase: with acetonitrile as mobile phase A and water as mobile phase B, perform gradient elution as specified in the table below:

Test spectra

Standard spectrogram

Sample profile

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